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Nepa Gene Co Ltd
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Shanghai GenePharma
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Sangon Biotech
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BioVector NTCC
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Image Search Results
Journal: Animals : an Open Access Journal from MDPI
Article Title: The Knockout of the ASIP Gene Altered the Lipid Composition in Bovine Mammary Epithelial Cells via the Expression of Genes in the Lipid Metabolism Pathway
doi: 10.3390/ani12111389
Figure Lengend Snippet: Construction of ASIP bMECs line: ( a ) gRNA’s location in the CDS region; ( b ) Sequencing map of positive bacterial liquid constructed by gASIP/PX459 vector; ( c ) ASIP knockout PCR validation of KO- ASIP MECs (M: market, 1: WT MEC, 2: KO- ASIP MEC); ( d ) Sequencing results of cell ASIP PCR products; ( e ) mRNA level validation of ASIP knockout (Ko- ASIP is an ASIP knockout bMECs. MEC is a wild-type bMECs. Error bars indicate SEM. * p < 0.05).
Article Snippet: Each sgRNA was inserted into the
Techniques: Sequencing, Construct, Plasmid Preparation, Knock-Out